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image analysis program imaris  (Oxford Instruments)


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    Oxford Instruments image analysis program imaris
    Image Analysis Program Imaris, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analysis+program+imaris/Imaris/pmc12482393-124-24-27
    Average 99 stars, based on 44185 article reviews
    image analysis program imaris - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Rab11 is essential to pancreas morphogenesis, lumen formation and endocrine mass
    Article Snippet: .. Whole-mount immunofluorescent images of pancreata stained for E-Cad and Muc1 were loaded into the image analysis program IMARIS. ..

    Article Title: Rab11 is essential to pancreas morphogenesis, lumen formation and endocrine mass
    Article Snippet: .. Whole-mount immunofluorescent images of pancreata stained for E-Cad and a lineage marker (Insulin & Glucagon; CPA1; Sox9) were loaded into the image analysis program IMARIS. .. A surface of a manually-determined Region of Interest (ROI) of E-Cad signal (detail level 2.5uM) was created to quantify the total volume of each pancreas (other parameters were determined automatically by IMARIS).

    Article Title: Rab11 is essential to pancreas morphogenesis, lumen formation and endocrine mass.
    Article Snippet: .. Pancreatic lineage volume quantifications Whole-mount immunofluorescent images of pancreata stained for ECad and a lineage marker (Insulin & Glucagon; CPA1; Sox9) were loaded into the image analysis program IMARIS. .. A surface of a manuallydetermined Region of Interest (ROI) of ECad signal (detail level 2.5 μm) was created to quantify the total volume of each pancreas (other parameters were determined automatically by IMARIS).

    Article Title: Rab11 is essential to pancreas morphogenesis, lumen formation and endocrine mass.
    Article Snippet: .. Lumen discontinuity quantifications Whole-mount immunofluorescent images of pancreata stained for ECad and Muc1 were loaded into the image analysis program IMARIS. ..

    Marker:

    Article Title: Rab11 is essential to pancreas morphogenesis, lumen formation and endocrine mass
    Article Snippet: .. Whole-mount immunofluorescent images of pancreata stained for E-Cad and a lineage marker (Insulin & Glucagon; CPA1; Sox9) were loaded into the image analysis program IMARIS. .. A surface of a manually-determined Region of Interest (ROI) of E-Cad signal (detail level 2.5uM) was created to quantify the total volume of each pancreas (other parameters were determined automatically by IMARIS).

    Article Title: Rab11 is essential to pancreas morphogenesis, lumen formation and endocrine mass.
    Article Snippet: .. Pancreatic lineage volume quantifications Whole-mount immunofluorescent images of pancreata stained for ECad and a lineage marker (Insulin & Glucagon; CPA1; Sox9) were loaded into the image analysis program IMARIS. .. A surface of a manuallydetermined Region of Interest (ROI) of ECad signal (detail level 2.5 μm) was created to quantify the total volume of each pancreas (other parameters were determined automatically by IMARIS).

    Laser-Scanning Microscopy:

    Article Title: Generation of a Rhesus Macaque induced Pluripotent Stem Cell Line (riPSC05) under Feeder-Free Conditions
    Article Snippet: Samples were mounted using ProLong TM Gold antifade reagent (Invitrogen P36934 ). .. Samples were imaged using an LSM880 (Zeiss) confocal laser scanning microscope and images were processed using the image analysis program IMARIS (Bitplane). ..

    Fluorescence:

    Article Title: In vivo validation of novel non-invasive PHP.eB AAVs as a potential therapeutic approach for alpha-synucleinopathies
    Article Snippet: .. For analysis of average fluorescence intensities for assays such as ascertaining the intensity of aSyn signal in the cells of the substantia nigra, the image analysis program Imaris was used, utilizing the “surfaces” tool. .. Imaris was also utilized to calculate transduction efficacy of the AAVs using the “spots” function, by creating spots for all GFP and NeuN (neuronal marker) or TH (dopaminergic neuron marker) and then using “colocalize spots” to find out how many cells had been penetrated by the GFP-expressing virus.

    Article Title: In vivo validation of novel non-invasive PHP.eB AAVs as a potential therapeutic approach for alpha-Synucleinopathies
    Article Snippet: .. For analysis of average fluorescence intensities for assays such as ascertaining the intensity of aSyn signal in the cells of the substantia nigra, the image analysis program Imaris was used, utilizing the “surfaces” tool. .. Imaris was also utilized to calculate transduction efficacy of the AAVs using the “spots” function, by creating spots for all GFP and NeuN (neuronal marker) or TH (dopaminergic neuron marker) and then using “colocalize spots” to find out how many cells had been penetrated by the GFP-expressing virus.



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    Fig. 2 The morphology of satellite and non-satellite microglia in the human orbitofrontal cortex after chronic TBI. A Representative <t>Imaris</t> 3D reconstructions of microglia (turquoise) and the apposed neuronal soma (red) for satellite microglia in control and severe TBI cases. These 3D reconstructions were used to calculate microglia, neuronal soma, and overlap volumes. B The volume of satellite microglia in control (ctl) and severe TBI (sT) cases (p = 0.7015, t(9) = 0.3832, paired t-test). C The volume of non-satellite microglia in control (ctl) and severe TBI (sT) cases (*p = 0.0181, t(9) = 2.883, paired t-test). D The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the microglial volume across groups (p = 0.0645, Wilcoxon test). E The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the neuronal soma across groups (p = 0.1055, Wilcoxon test). F Representative Imaris reconstruction of the soma (blue) and filaments (grey) used to analyze branching in microglia. G Scholl <t>analysis</t> plot of satellite microglia in control (black) and severe TBI (red) conditions (p = 0.5206, F(1,18) = 0.4293, for TBI effect and 0.9832, F(40,640) = 0.5782, for distance x TBI effect, mixed-effects analysis). H Scholl analysis plot of non-satellite microglia in control (black) and severe TBI (red) conditions (p = 0.7120, F(1,18) = 0.1406, for TBI effect and 0.2347, F(40,640) = 1.160, for distance x TBI effect, mixed-effects analysis). One reconstructed microglia per case is represented with a symbol; solid lines indicate the mean ± SEM in B,C,D,E. In G and H, the mean for each distance is represented by a symbol. (n = 10 control and sTBI cases that are age- and sex- matched across groups)
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    Fig. 2 The morphology of satellite and non-satellite microglia in the human orbitofrontal cortex after chronic TBI. A Representative <t>Imaris</t> 3D reconstructions of microglia (turquoise) and the apposed neuronal soma (red) for satellite microglia in control and severe TBI cases. These 3D reconstructions were used to calculate microglia, neuronal soma, and overlap volumes. B The volume of satellite microglia in control (ctl) and severe TBI (sT) cases (p = 0.7015, t(9) = 0.3832, paired t-test). C The volume of non-satellite microglia in control (ctl) and severe TBI (sT) cases (*p = 0.0181, t(9) = 2.883, paired t-test). D The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the microglial volume across groups (p = 0.0645, Wilcoxon test). E The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the neuronal soma across groups (p = 0.1055, Wilcoxon test). F Representative Imaris reconstruction of the soma (blue) and filaments (grey) used to analyze branching in microglia. G Scholl <t>analysis</t> plot of satellite microglia in control (black) and severe TBI (red) conditions (p = 0.5206, F(1,18) = 0.4293, for TBI effect and 0.9832, F(40,640) = 0.5782, for distance x TBI effect, mixed-effects analysis). H Scholl analysis plot of non-satellite microglia in control (black) and severe TBI (red) conditions (p = 0.7120, F(1,18) = 0.1406, for TBI effect and 0.2347, F(40,640) = 1.160, for distance x TBI effect, mixed-effects analysis). One reconstructed microglia per case is represented with a symbol; solid lines indicate the mean ± SEM in B,C,D,E. In G and H, the mean for each distance is represented by a symbol. (n = 10 control and sTBI cases that are age- and sex- matched across groups)
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    Image Search Results


    Fig. 2 The morphology of satellite and non-satellite microglia in the human orbitofrontal cortex after chronic TBI. A Representative Imaris 3D reconstructions of microglia (turquoise) and the apposed neuronal soma (red) for satellite microglia in control and severe TBI cases. These 3D reconstructions were used to calculate microglia, neuronal soma, and overlap volumes. B The volume of satellite microglia in control (ctl) and severe TBI (sT) cases (p = 0.7015, t(9) = 0.3832, paired t-test). C The volume of non-satellite microglia in control (ctl) and severe TBI (sT) cases (*p = 0.0181, t(9) = 2.883, paired t-test). D The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the microglial volume across groups (p = 0.0645, Wilcoxon test). E The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the neuronal soma across groups (p = 0.1055, Wilcoxon test). F Representative Imaris reconstruction of the soma (blue) and filaments (grey) used to analyze branching in microglia. G Scholl analysis plot of satellite microglia in control (black) and severe TBI (red) conditions (p = 0.5206, F(1,18) = 0.4293, for TBI effect and 0.9832, F(40,640) = 0.5782, for distance x TBI effect, mixed-effects analysis). H Scholl analysis plot of non-satellite microglia in control (black) and severe TBI (red) conditions (p = 0.7120, F(1,18) = 0.1406, for TBI effect and 0.2347, F(40,640) = 1.160, for distance x TBI effect, mixed-effects analysis). One reconstructed microglia per case is represented with a symbol; solid lines indicate the mean ± SEM in B,C,D,E. In G and H, the mean for each distance is represented by a symbol. (n = 10 control and sTBI cases that are age- and sex- matched across groups)

    Journal: Journal of neuroinflammation

    Article Title: Satellite microglia: marker of traumatic brain injury and regulator of neuronal excitability.

    doi: 10.1186/s12974-024-03328-9

    Figure Lengend Snippet: Fig. 2 The morphology of satellite and non-satellite microglia in the human orbitofrontal cortex after chronic TBI. A Representative Imaris 3D reconstructions of microglia (turquoise) and the apposed neuronal soma (red) for satellite microglia in control and severe TBI cases. These 3D reconstructions were used to calculate microglia, neuronal soma, and overlap volumes. B The volume of satellite microglia in control (ctl) and severe TBI (sT) cases (p = 0.7015, t(9) = 0.3832, paired t-test). C The volume of non-satellite microglia in control (ctl) and severe TBI (sT) cases (*p = 0.0181, t(9) = 2.883, paired t-test). D The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the microglial volume across groups (p = 0.0645, Wilcoxon test). E The ratio of the overlap volume between satellite microglia and the apposing neuronal soma to the neuronal soma across groups (p = 0.1055, Wilcoxon test). F Representative Imaris reconstruction of the soma (blue) and filaments (grey) used to analyze branching in microglia. G Scholl analysis plot of satellite microglia in control (black) and severe TBI (red) conditions (p = 0.5206, F(1,18) = 0.4293, for TBI effect and 0.9832, F(40,640) = 0.5782, for distance x TBI effect, mixed-effects analysis). H Scholl analysis plot of non-satellite microglia in control (black) and severe TBI (red) conditions (p = 0.7120, F(1,18) = 0.1406, for TBI effect and 0.2347, F(40,640) = 1.160, for distance x TBI effect, mixed-effects analysis). One reconstructed microglia per case is represented with a symbol; solid lines indicate the mean ± SEM in B,C,D,E. In G and H, the mean for each distance is represented by a symbol. (n = 10 control and sTBI cases that are age- and sex- matched across groups)

    Article Snippet: We acknowledge the NIH S10 OD016240 Grant that funded the Leica SP8X confocal microscope and the UW student technology fee that funded the computer, scanning hardware, and deconvolution software for the microscope and the IMARIS image analysis program.

    Techniques: Control